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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/01/2006 |
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Data da última atualização: |
27/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
REMUSKA, A. C.; JACCOUD FILHO, D. S.; CATTELAN, A. J.; MICHEREFF, S.; TESSMANN, D. J. |
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Afiliação: |
ALEXANDRE JOSE CATTELAN, CNPSO. |
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Título: |
Comparative studies between common bean seeds treatments by using antagonistic microorganims, chemical and biological fungicides in arder to contrai the fungus Colletotrichum lindemuthianum and their influence in promoting the plants's growth. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: SEED HEALTH SYMPOSIUM, 5., 2005, Angers. Abstract booklet. Angers: INRA: ISTA, 2005. |
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Páginas: |
p. 44-45. |
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Idioma: |
Inglês |
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Notas: |
Resumo n. 58. |
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Conteúdo: |
This study aimed to compare the efficiency of treatments common bean (Phaseolus vulgaris L.) seeds by using a chemical fungicide (Carbendazim, Derosal), biological fungicides (Agrotich and Biotrichl) and antagonistic microorganisms (Trichoderma spp., Pseudomonas from fluorescent group and Bacillus subtilis) in order to control the fungus Colletotrichum lindemuthianum and their influence in promoting plant growth. For the evaluation of the antagonism in vitro it was used the technique of colony pairing between antagonists and C. lindemuthianum. For the evaluation of the biological fungicides it was used the technique in which the products containing the Trichoderma spp. propagules were put in contact with the C. lindemuthianum at zero and five days after its transfer to the PDA media. The tested doses were 0 g/l-¹ (control Petri dish), 1 g/l-¹, 2 g/l-¹, 3 g/l-¹ and 4 g/l-¹. The evaluation of in vitro antagonism was done by the microbiolization process of the seeds (artificially infected with C. lindemuthianum) with antagonist microorganism as well as with the chemical and biological fungicides. The seeds were sowed in pots containing sterilized soil. Analysis were carried out in the tenth and twentieth D.A.S. of the disease rate, lesions diameter and on the thirtieth D.A.S evaluations of the plants height, dry and fresh mass of aerial part of the rool. The Pseudomonas isolate from fluorescent group presented inhibition percentage of 26,3% to 34,8%. B. subtilis presented 51,3% of the inhibition in the plant pathogen colony growth. The Trichoderma spp. isolate inhibition percentage range from 31,1 % (T. harzianum) to 59,1% (T. koningii). The isolates of Trichoderma spp. and B. subtilis were more promising in the in vitro control of C. lindemuthianum. Under greenhouse conditions, there were no significantly differences between plant growth promotion. T. koningii and Pseudomonas from fluorescent group as well as the biological fungicide Biotrich were able to present diseases rates similar to the standard fungicide Derosal. Other isolates, presented a tendency to decrease the disease, and probably they could be used as chemical fungicide auxiliary to seeds treatment in arder to control diseases. MenosThis study aimed to compare the efficiency of treatments common bean (Phaseolus vulgaris L.) seeds by using a chemical fungicide (Carbendazim, Derosal), biological fungicides (Agrotich and Biotrichl) and antagonistic microorganisms (Trichoderma spp., Pseudomonas from fluorescent group and Bacillus subtilis) in order to control the fungus Colletotrichum lindemuthianum and their influence in promoting plant growth. For the evaluation of the antagonism in vitro it was used the technique of colony pairing between antagonists and C. lindemuthianum. For the evaluation of the biological fungicides it was used the technique in which the products containing the Trichoderma spp. propagules were put in contact with the C. lindemuthianum at zero and five days after its transfer to the PDA media. The tested doses were 0 g/l-¹ (control Petri dish), 1 g/l-¹, 2 g/l-¹, 3 g/l-¹ and 4 g/l-¹. The evaluation of in vitro antagonism was done by the microbiolization process of the seeds (artificially infected with C. lindemuthianum) with antagonist microorganism as well as with the chemical and biological fungicides. The seeds were sowed in pots containing sterilized soil. Analysis were carried out in the tenth and twentieth D.A.S. of the disease rate, lesions diameter and on the thirtieth D.A.S evaluations of the plants height, dry and fresh mass of aerial part of the rool. The Pseudomonas isolate from fluorescent group presented inhibition percentage of 26,3% to 34,8%. B. subtilis presented 51,3%... Mostrar Tudo |
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Thesagro: |
Feijão; Phaseolus Vulgaris. |
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Thesaurus Nal: |
Beans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03058nam a2200217 a 4500
001 1468771
005 2020-02-27
008 2005 bl uuuu u00u1 u #d
100 1 $aREMUSKA, A. C.
245 $aComparative studies between common bean seeds treatments by using antagonistic microorganims, chemical and biological fungicides in arder to contrai the fungus Colletotrichum lindemuthianum and their influence in promoting the plants's growth.$h[electronic resource]
260 $aIn: SEED HEALTH SYMPOSIUM, 5., 2005, Angers. Abstract booklet. Angers: INRA: ISTA$c2005
300 $ap. 44-45.
500 $aResumo n. 58.
520 $aThis study aimed to compare the efficiency of treatments common bean (Phaseolus vulgaris L.) seeds by using a chemical fungicide (Carbendazim, Derosal), biological fungicides (Agrotich and Biotrichl) and antagonistic microorganisms (Trichoderma spp., Pseudomonas from fluorescent group and Bacillus subtilis) in order to control the fungus Colletotrichum lindemuthianum and their influence in promoting plant growth. For the evaluation of the antagonism in vitro it was used the technique of colony pairing between antagonists and C. lindemuthianum. For the evaluation of the biological fungicides it was used the technique in which the products containing the Trichoderma spp. propagules were put in contact with the C. lindemuthianum at zero and five days after its transfer to the PDA media. The tested doses were 0 g/l-¹ (control Petri dish), 1 g/l-¹, 2 g/l-¹, 3 g/l-¹ and 4 g/l-¹. The evaluation of in vitro antagonism was done by the microbiolization process of the seeds (artificially infected with C. lindemuthianum) with antagonist microorganism as well as with the chemical and biological fungicides. The seeds were sowed in pots containing sterilized soil. Analysis were carried out in the tenth and twentieth D.A.S. of the disease rate, lesions diameter and on the thirtieth D.A.S evaluations of the plants height, dry and fresh mass of aerial part of the rool. The Pseudomonas isolate from fluorescent group presented inhibition percentage of 26,3% to 34,8%. B. subtilis presented 51,3% of the inhibition in the plant pathogen colony growth. The Trichoderma spp. isolate inhibition percentage range from 31,1 % (T. harzianum) to 59,1% (T. koningii). The isolates of Trichoderma spp. and B. subtilis were more promising in the in vitro control of C. lindemuthianum. Under greenhouse conditions, there were no significantly differences between plant growth promotion. T. koningii and Pseudomonas from fluorescent group as well as the biological fungicide Biotrich were able to present diseases rates similar to the standard fungicide Derosal. Other isolates, presented a tendency to decrease the disease, and probably they could be used as chemical fungicide auxiliary to seeds treatment in arder to control diseases.
650 $aBeans
650 $aFeijão
650 $aPhaseolus Vulgaris
700 1 $aJACCOUD FILHO, D. S.
700 1 $aCATTELAN, A. J.
700 1 $aMICHEREFF, S.
700 1 $aTESSMANN, D. J.
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Registro original: |
Embrapa Soja (CNPSO) |
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Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
15/02/1993 |
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Data da última atualização: |
16/08/2022 |
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Autoria: |
JAUME, C. M.; CAMPOS, A. L. |
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Afiliação: |
CNPGL. |
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Título: |
Criopreservação de embriões de camundongos e bovinos utilizando metanol como crioprotetor. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v. 25, n. 1, p. 9-13, 1990. |
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Idioma: |
Português |
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Conteúdo: |
RESUMO - Embriões de camundongos coletados no quarto dia após a injeção de I-ICG foram colocados diretamente em uma solução 3 M de metanol em P85 suplementado com 10% de soro fetal bovino (P855), à temperatura ambiente, durante dez minutos. Foram acondicionados em "paillets" de 0,25 ml e colocados diretamente a - 10 0C durante cinco minutos antes e durante seis minutos depois de induzir a formação de gelo. O descongelainento foi realizado em banho-maria a 370C durante 20 segundos. Os embriões foram colocados diretamente em P855, lavados duas vezes e colocados em cultura utilizando meio T 6suplementado com 10% de soro fetal bovino, a 370C numa atmosfera umedecida dc 5% CO2, 5% 02 e 90% N2. Os embriões bovinos foram coletados de fêmeas superovuladas, sete dias após o cio, e processados da mesma maneira que os embriões de camundongo. Dos 104 embriões de camundongo criopreservados e descongelados, 48,2% continuaram seu desenvolvimento cm cultura. Dos 20 embriões bovinos criopreservados e descongelados, oito foram transferidos diretamente a receptoras, e doze foram colocados em cultura. Nenhuma das receptoras ficou gestante e nenhum dos embriões continuou seu desenvolvimento em cultura. ABSTRACT - Mouse embryos were collccted from superovulated animais on the fourth day after IICG injection. They were put directly into a solution of 3 M methanol in PBSS at room temperature during ten minutes. Thawing was carried out in a waterbatb at 37 0C during 20seconds. Embryos were placed directly into PBSS washed twice and put into culture using medium T6supplemented with 10% fetal calf serum at 37 °C in a humid atmosphere of 5% CO2 , 5% 02 and 90% N2 . Bovine embryos were cultured under the same conditions, only using PBS supplemented with 20% fetal calf serum as culture medium. Of the 104 frozen mouse embryos, 48,2% continued developing in culture after thawing. None of the eight bovine embryos that were thawed and transferred directly to recipients resulted in pregnancy and none of the twelve bovine embryos thatwere thawed and put into culture continued to develop. MenosRESUMO - Embriões de camundongos coletados no quarto dia após a injeção de I-ICG foram colocados diretamente em uma solução 3 M de metanol em P85 suplementado com 10% de soro fetal bovino (P855), à temperatura ambiente, durante dez minutos. Foram acondicionados em "paillets" de 0,25 ml e colocados diretamente a - 10 0C durante cinco minutos antes e durante seis minutos depois de induzir a formação de gelo. O descongelainento foi realizado em banho-maria a 370C durante 20 segundos. Os embriões foram colocados diretamente em P855, lavados duas vezes e colocados em cultura utilizando meio T 6suplementado com 10% de soro fetal bovino, a 370C numa atmosfera umedecida dc 5% CO2, 5% 02 e 90% N2. Os embriões bovinos foram coletados de fêmeas superovuladas, sete dias após o cio, e processados da mesma maneira que os embriões de camundongo. Dos 104 embriões de camundongo criopreservados e descongelados, 48,2% continuaram seu desenvolvimento cm cultura. Dos 20 embriões bovinos criopreservados e descongelados, oito foram transferidos diretamente a receptoras, e doze foram colocados em cultura. Nenhuma das receptoras ficou gestante e nenhum dos embriões continuou seu desenvolvimento em cultura. ABSTRACT - Mouse embryos were collccted from superovulated animais on the fourth day after IICG injection. They were put directly into a solution of 3 M methanol in PBSS at room temperature during ten minutes. Thawing was carried out in a waterbatb at 37 0C during 20seconds. Embryos were placed di... Mostrar Tudo |
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Thesagro: |
Bovino; Camundongo; Criopreservação; Embrião; Metanol; Soro. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/590394/1/Criopreservacao-de-embrioes-de-camundongos-e-bovinos-utilizando-metanol-como-crioprotetor.pdf
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Marc: |
LEADER 02700naa a2200205 a 4500
001 1590394
005 2022-08-16
008 1990 bl uuuu u00u1 u #d
100 1 $aJAUME, C. M.
245 $aCriopreservação de embriões de camundongos e bovinos utilizando metanol como crioprotetor.$h[electronic resource]
260 $c1990
520 $aRESUMO - Embriões de camundongos coletados no quarto dia após a injeção de I-ICG foram colocados diretamente em uma solução 3 M de metanol em P85 suplementado com 10% de soro fetal bovino (P855), à temperatura ambiente, durante dez minutos. Foram acondicionados em "paillets" de 0,25 ml e colocados diretamente a - 10 0C durante cinco minutos antes e durante seis minutos depois de induzir a formação de gelo. O descongelainento foi realizado em banho-maria a 370C durante 20 segundos. Os embriões foram colocados diretamente em P855, lavados duas vezes e colocados em cultura utilizando meio T 6suplementado com 10% de soro fetal bovino, a 370C numa atmosfera umedecida dc 5% CO2, 5% 02 e 90% N2. Os embriões bovinos foram coletados de fêmeas superovuladas, sete dias após o cio, e processados da mesma maneira que os embriões de camundongo. Dos 104 embriões de camundongo criopreservados e descongelados, 48,2% continuaram seu desenvolvimento cm cultura. Dos 20 embriões bovinos criopreservados e descongelados, oito foram transferidos diretamente a receptoras, e doze foram colocados em cultura. Nenhuma das receptoras ficou gestante e nenhum dos embriões continuou seu desenvolvimento em cultura. ABSTRACT - Mouse embryos were collccted from superovulated animais on the fourth day after IICG injection. They were put directly into a solution of 3 M methanol in PBSS at room temperature during ten minutes. Thawing was carried out in a waterbatb at 37 0C during 20seconds. Embryos were placed directly into PBSS washed twice and put into culture using medium T6supplemented with 10% fetal calf serum at 37 °C in a humid atmosphere of 5% CO2 , 5% 02 and 90% N2 . Bovine embryos were cultured under the same conditions, only using PBS supplemented with 20% fetal calf serum as culture medium. Of the 104 frozen mouse embryos, 48,2% continued developing in culture after thawing. None of the eight bovine embryos that were thawed and transferred directly to recipients resulted in pregnancy and none of the twelve bovine embryos thatwere thawed and put into culture continued to develop.
650 $aBovino
650 $aCamundongo
650 $aCriopreservação
650 $aEmbrião
650 $aMetanol
650 $aSoro
700 1 $aCAMPOS, A. L.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv. 25, n. 1, p. 9-13, 1990.
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